Glycyrrhizic acid suppresses early stage of adipogenesis through repression of MEK/ERK-mediated C/EBP? and C/EBP? expression in 3T3-L1 cells

Glycyrrhizic acid (GA), a major constituent of the root licorice (Glycyrrhiza glabra), and has various biological activities, including anti-obesity property. However, molecular mechanism anti-adipogenic effect GA is still unclear. In this study, we investigated effects in mouse adipocytic 3T3-L1 cells elucidated its underlying mechanism. decreased intracellular triglyceride level. The expression levels adipogenic lipogenic genes were lowered by treatment with concertation-dependent manner. contrast, did not affect lipolytic gene released glycerol suppressed early stage adipogenesis when it was added for 0–3 h after initiation adipogenesis. Moreover, reduced mRNA CCAAT/enhancer binding protein (C/EBP) ? C/EBP?, both which activate Furthermore, phosphorylation extracellular signal-regulated kinase [ERK: p44/42 mitogen-activated (MAPK)] addition, MAPK (MEK) inhibitor, PD98059 C/EBP? C/EBP? expression. These results indicate that through repressing MEK/ERK-mediated cells. Thus, an ability possible agent obesity.